Study of correlation between serum vit-B12 and homocysteine levels with ischemic heart disease

 

 

Abstract              Background: Vitamin B12 reduces the risk of coronary heart disease and stroke by quenching homocysteine. Hyperhomocysteinemia, is emerging as a strong risk factor for cardiovascular events. Aim: To find out the correlation between serum vit-B12 and homocysteine levels with ischemic heart disease. Material and Methods: A total of 100 patients of Ischemic Heart Disease (IHD) were included as cases and 150 normal healthy populations was selected as controls. Quantitative determination of total L-homocysteine in human serum or plasma was done by using Chemiluminescent Microparticle Immunoassay (CIMA) technology and vitamin B12 assay estimation was done by competitive binding immunoenzymatic assay. Results: Out of 100 IHD patients, 59 had low vitamin B12 levels and 69 out of 150 individuals in control group had low vitamin B12 levels (p<0.05). The number of individuals with high homocysteine levels was 75 out of 100 in patient group and 79 out of 150 in control group. 25 cases were with normal vit B12 levels and high homocysteine and 22 controls with normal vit B12 levels and high homocysteine levels. Hyperhomocysteinemia was found even amongst those with normal vit B12 levels (p>0.05). Conclusion: Low vit B12 and high homocysteine levels are significantly associated with IHD.

Key Words: Ischemic heart disease, serum vit-B12, homocysteine, correlation