Table of Content - Volume 11 Issue 2 - August 2018
Study of lipid profile of diabetic and nondiabetic young stroke patients
S P Toshniwal1, S Yadav2, A Dhakne3*, A Khan4
1Assistant Professor, 2Senior Resident, 3,4Junior Resident, Department of Medicine, Government Medical College, Latur, Maharashtra, INDIA Email: dr_shamt@rediffmail.com
Abstract Background: The diagnosis of diabetes in a stroke patient would change the initial management of that patient, specifically with respect to other risk factors like lipid and blood pressure management. Aim: To study lipid profile in diabetic and non-diabetic newly diagnosed young stroke patients. Material and Methods: A total of 138 patients were admitted during study period were selected by simple random sampling with acute stroke in the study. Fasting total cholesterol, triglycerides, high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were measured from venous blood samples. Results: The mean HbA1c for group D and group ND were 9.04±1.02 and 4.85±0.45 respectively. This difference in HbA1c among patients in two groups was statistically significant. The mean values of LDL, VLDL, total cholesterol and triglycerides showed significant association between two groups. Levels of HDL were significantly lower in Group D as compared to Group ND. Conclusion: The lipid profile of LDL, VLDL, total cholesterol and triglycerides showed significant higher range in diabetic patients as compared to non- diabetics while HDL was lower in diabetic patients as compared to non- diabetics. Key Words: Young adults, diabetic, non-diabetic, lipid profile
INTRODUCTION Diabetes mellitus remains an independent risk factor of stroke and coronary heart disease. The increased risk of recurrent stroke due to diabetes ranges from 2.1 to 5.6 times that of non-diabetic patients and is independent of glucose control during interstroke period.1 Approximately one-third of all patients with diabetes have undiagnosed diabetes (i.e. not recognized by their clinician) and usually present as complications like stroke, myocardial infarction and diabetic foot.2 The under diagnosis of diabetes in the general population together with the strong association of diabetes with stroke suggest a rationale for screening all hyperglycemic stroke patients for diabetes. The diagnosis of diabetes in a stroke patient would change the initial management of that patient, specifically with respect to other risk factors like lipid and blood pressure management. It is thought that in young patients the common risk factors of stroke such as smoking, diabetes and hypertension are additive to coagulopathies, infectious causes of stroke and acquired and congenital heart diseases, and therefore these patients may have a different clinical and metabolic profile. Since, there is a paucity of local data regarding undiagnosed diabetes in young stroke patients, the current study was conducted to study lipid profile in diabetic and non-diabetic newly diagnosed young stroke patients.
MATERIAL AND METHODS The present study was cross sectional study carried out at Tertiary Institute to study lipid profile in diabetic and non-diabetic newly diagnosed young stroke patients. All diabetic and non-diabetic newly diagnosed young stroke cases admitted at tertiary care center during period of study were included. The study was conducted after obtaining clearance from the Ethical Committee of the institute and permission from the appropriate authority. Sample size A total of 138 patients were admitted during study period were selected by simple random sampling satisfying inclusion and exclusion criteria with acute stroke in the study. Inclusion criteria
Exclusion criteria
Selected patients are subjected for the following investigations: FBS / PPBS /HbA1c, lipid profile, Serum electrolytes, ECG and ECHO cardiography (as required).CT Brain was done and MRI Brain (if CT brain showed normal study). Serum Homocystein, Protein C and S, APLA tests were done as required.Data were collected through interview and review of medical records. Participant’s age, sex, smoking status and physical activity were obtained through interview. Anthropometric measurements The height of each participant was measured without shoes using a mounted stadiometer to the nearest 0.1cm. The participants were weighed without heavy clothing to the nearest 0.1kg using a digital scale. Body mass index was then calculated as the ratio of weight in kilogram (kg) to height in meter squared (m2). Participants were defined as obese in accordance with world health organization criteria; The rest of the participants were classified as overweight (25.0–29.9 kg/m2), normal (18.5–24.9 kg/m2) and underweight (<18.5 kg/m2).WC was measured in the horizontal plane at the superior border of the right iliac crest. WC were measured to the nearest 0.1 cm at the end of a normal expiration. Before recording the measurement, it was ensured that the tape was snug but did not compress the skin and was parallel to the floor.
Laboratory assays Fasting total cholesterol, triglycerides, high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) were measured from venous blood samples. Following overnight fast (> 8 hours without food), venous blood sample was drawn by the attending clinician in a private consulting room from the brachial vein into gel separator tubes. Blood samples were kept at room temperature for 2 hours before centrifuging for 5 minutes at 3000 rpm to separate serum from cellular elements. Serum was collected into microeppendorff tubes and stored at -80°C until processed. The fully automated Cobas1 C501/502 (Roche) system was used for determining serum lipid profiles. Statistical analysis The statistical analyses performed using the Statistical Package for Social Science (SPSS) version 21 for Windows. Data were expressed as mean values ± standard deviations (SD) for continuous variables. Frequency and proportions were reported for categorical variables. The p-value of < 0.05 was considered statistically significant.
RESULTS It was observed that majority of patients in Group D were in age group 36-45 years. 21 (46.66%), similarly patients in Group ND were in age group 36-45 years (58.06%). The mean age in group D was 34.06 ±8.35years and group ND was37.44 ±8.55 years. There was significant difference in age distribution in all two groups. (p<0.05). 9 (20%) of the diabetics were diagnosed as diabetics for the first time during their hospital admission in this study. The mean age in group D, males was 35.03±8.21 years and females were 31.41±8.53 years. The mean age in group ND males was 37.60±8.87 years and females were 37.35±8.55 years with no statistical difference. (P>0.05). Out of total 130 patients, 93 were males while 45 were females. There were 33 (73.33%) and 60 (64.52%) male patients in Group D and Group ND respectively. There were 12 (26.67%) and 33 (35.48%) females in Group D and Group ND respectively. There was no gender difference when two groups were compared statistically. (p>0.05). Table 1: Distribution according to anthropometric characteristics
(P values by t test; P<0.05 statistically significant)
It was observed that 26 (57.77%) and 58 (62.36%) patients had Waist Circumference (>90cm males, >80cm females) in Group D and Group ND respectively. Overweight and obesity (BMI >25 kg/m2) was observed in 22 (48.89%) and 46 (49.46%) patients in Group D and Group ND respectively. The mean waist circumference in Group D and Group ND was 90.96±9.60 and 87.34±7.24 cm respectively with statistically significant difference in waist circumference distribution in two groups. (p<0.05).The mean BMI circumference in Group D and Group ND was 26.87±4.50 and 24.67±3.66 kg/m2 respectively with statistically significant difference in waist circumference distribution in two groups. (p<0.05).It was observed that 36 (80%) and 72 (77.42%) patients had ischemic stroke in Group D and Group ND respectively. Hemorrhagic stroke was observed in 9 (20%) and 21(22.58%) patients in Group D and Group ND respectively. The differences were not statistically significant. (p>0.05).
Table 2: Distribution according to lipid profile
(P values by t test; P<0.05 statistically significant) It was observed that mean values of LDL, VLDL, total cholesterol and triglycerides showed significant association between two groups. (p<0.05). Levels of HDL were significantly lower in Group D as compared to Group ND. (p<0.05).
Table 3: Distribution according to blood glucose levels
(P<0.001 Statistically highly Significant by t-test) The mean fasting blood glucose levels in patients in Group D was 163.18 ±15.60 and in Group ND was 92.06 ±10.51. This difference in fasting blood glucose among patients in two groups was statistically significant. (P<0.05).The mean postprandial blood glucose level in patients in Group D was301.37 ±53.14 and in Group ND was 164.13 ±15.89. This difference in postprandial blood glucose among patients in two groups was statistically significant. (P<0.05). The mean HbA1c for group D and group ND were 9.04±1.02 and 4.85±0.45 respectively. This difference in HbA1c among patients in two groups was statistically significant. (P<0.05).
DISCUSSION The mean waist circumference in Group D and Group ND was 90.96 ±9.60 and 87.34 ±7.24 cm respectively with statistically significant difference in waist circumference distribution in two groups. (p<0.05) The mean BMI in Group D and Group ND was 26.87 ±4.50 and 24.67 ±3.66 kg/m2 respectively with statistically significant difference in BMI distribution in two groups. (p<0.05). Bosnar-Puretić M et al3 assessed the presence of obesity among other risk factors for stroke in younger adult patients with ischemic stroke. It was a pilot study performed in ischemic stroke patients aged 18-55. The mean waist circumference was 94.9 +/- 5.8 cm in the control group and 102.6 +/- 9.8 cm in the male stroke group. There was significant difference in waist circumference between the control and patient groups. In younger males, waist circumference could be considered as an important risk factor for stroke. These values were based on western population. Mitchell et al4 in a population-based case-control study investigated the relationship of obesity and young-onset ischemic stroke. Stroke cases were between the ages of 15 and 49. In analyses adjusted for age, sex, and ethnicity, obesity (BMI > 30 kg/m2) was associated with an increased stroke risk (odds ratio, 1.57, 95% C.I. = 1.28–1.94) These results indicate that obesity is a risk factor for young onset ischemic stroke. Jood K et al5 in a population- based prospective study over 28 years, increased BMI in mid-life was found to be associated with an increased risk for ischemic and unspecified stroke, but not with hemorrhagic stroke. The result supports the role of mid-life BMI as a risk factor for stroke in later life and suggests a differentiated effect on stroke subtypes. High BMI has been found to be a risk factor for both ischemic and hemorrhagic stroke in people of 40 to 64 years by another prospective study by Song YM et al.6The distribution of patients according to type of stroke showed that 36 (80%) and 72 (77.42%) patients had ischemic stroke in Group D and Group ND respectively. Hemorrhagic stroke was observed in 9 (20%) and 21 (22.58%) patients in Group D and Group ND respectively. It was observed that major cause of ischemic stroke was small artery occlusion 10 (27.78%) in diabetes group and hypertension for hemorrhagic type 7(77.78%) Cardio aortic embolism was major ischemic type for Group ND 18 (25%) Eclampsia case showed hemorrhagic stroke while peripartum / postpartum / OC pill user patients all had venous sinus thrombosis and venous infarct. Jadhav et al7 studied the clinical profile and risk factors associated with the stroke in young adults between 15-45 years. Out of 40 patients 12 (30%) reported embolic stroke while 8 (20%) reported stroke with CVST. Thrombotic and haemorrhagic stroke were observed in 10 (25%) patients each. Harsha Kumar et al100 in retrospective, record-based study of patients of stroke in the age group of 15-45 years observed out of the 109 cases of stroke, 61 (56%) were ischemic stroke, 25 (22.9%) were hemorrhagic stroke and 23 (21.1%) were embolic stroke. In a study in young stroke patients by Nayak SD et al8 thrombotic stroke and cardio-embolic stroke occurred in 24% and 17% patients respectively. Overall, there is a male preponderance of stroke. Studies performed on ischemic stroke among the 15-45 years age group from India also reported a male preponderance. In several studies, females outnumbered men among those under 30.9-11 Zafar et al12 studied pattern of stroke in type 2 diabetic subjects versus non diabetic subjects observed out of 50 diabetic patients, 44 (88.0%) had ischemic stroke and 6 (12.0%) had intracerebral haemorrhage. In non- diabetics, 29 (58.0%) had ischemic stroke while 21 (42.0%) had intracerebral haemorrhage. On further analysis of ischemic stroke, cortical infarcts (CI) was found in 22, sub cortical infarcts (SCI) in 14, brainstem in 5 and cerebellar in 2 diabetic patients. CI was also the commonest subtype of ischemic stroke in nondiabetics. Singh et al13 studied clinical profile of stroke in relation to glycaemic status of patients and observed 75% of cases of strokes in the euglycemic group presented as ischemia (15 out of 20) and five cases in the same group occurred as haemorrhage (5 out of 15). All stress hyperglycaemic strokes were haemorrhagic (100%), and also all the strokes occurred in the new diabetic group were haemorrhagic strokes (100%). 85.71% of stroke in the known diabetic group were haemorrhagic strokes (12 out of 14). This shows that haemorrhagic strokes occurred in the hyperglycaemic patients, maximum being in the stress hyperglycaemia and new diabetics. It was observed that LDL, VLDL, HDL, total cholesterol and triglycerides showed significant association between two groups. Mean levels of LDL, VLDL triglycerides and total cholesterol were significantly greater in group D as compared to Group ND. The mean HDL values of group D was significantly lower than that in Group ND. (P<0.05). Mishra et al14 in a cross sectional study performed on 64 stoke patients after dividing in to group 1 (age <40 years) and group 2 (>40 years) on clinical profile and risk factors stroke with special reference to lipid profile observed group 1 and Group 2, raised cholesterol, raised triglyceride, raised LDL and low HDL was recorded in 30% versus 42.55%, 30% versus 31.48%, 40% versus 68.45% and 60% versus 27.75% of patients respectively. Bhaskar et al15 also studied the association between serum lipid level in patients with stoke and reported a strong association between LDL-C and atherosclerosis development. Rai et al16 studied serum lipid profile in stroke patients in Northern India and observed that mean TC and LDL-C levels were significantly much higher in the ischemic stroke patients when compared to patients with controls (183.7±34.5 versus 148.5±30.6 and 118.7±26.7 versus 81.4±22.0). This was similar to the present study results. Sreedhar et al17 in a study on lipid profile in Non-Diabetic Stroke observed positive correlation between serum Total cholesterol, Triglycerides, LDL levels and risk of stroke. The mean fasting blood glucose levels in patients in Group D was 163.18 ± 15.60 and in Group ND was 92.06 ±10.51. This difference in fasting blood glucose among patients in two groups was statistically significant. (P<0.05). The mean postprandial blood glucose level in patients in Group D was 301.37 ±53.14 and in Group ND was 164.13 ±15.89. This difference in postprandial blood glucose among patients in two groups was statistically significant. (P<0.05). The mean HbA1c for group D and group ND were 9.04±1.02 and 4.85±0.45 respectively. This difference in HbA1c among patients in two groups was statistically significant. (P<0.05).Hyperglycemia is common in patients with acute stroke, occurring in upto 60% of patients and is believed to aggravate cerebral ischemia. It leads to intracellular acidosis, accumulation of extra cellular Glutamate, cerebral oedema, blood-brain barrier disruption, and tendency for haemorrhagic transformation. It is observed that between 20-40% of patients admitted with ischemic stroke are hyperglycemic, often without a pre-existing diagnosis of diabetes, which can be due to stress hyperglycemia or undiagnosed diabetes exposed during an acute incident. HbA1c was done in all patients to rule out stress hyperglycemia. Zahra et al18 determine the frequency of newly diagnosed diabetes mellitus in acute ischemic stroke patients and observed average fasting blood sugar in diabetic subjects was 148±10 mg/dl which was significantly high as compared to non-diabetic patients.
CONCLUSION The lipid profile of LDL, VLDL, total cholesterol and triglycerides showed significant higher range in diabetic patients as compared to non-diabetics while HDL was lower in diabetic patients as compared to non- diabetics. REFERENCES
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